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Objective: To evaluate different doses of ivermectin in adult patients with mild COVID-19 and to evaluate the effect of ivermectin on mortality and clinical consequences. Methods: A randomized, double-blind, placebo-controlled, multicenter clinical trial was performed at five hospitals. A total of 180 mild hospitalized patients with COVID-19 confirmed by PCR or chest image tests were enrolled and allocated to six arms including hydroxychloroquine 200 mg twice per day, placebo plus hydroxychloroquine 200 mg twice per day, single dose ivermectin (200 μg/kg), three low interval doses of ivermectin (200, 200, 200 μg/kg), single dose ivermectin (400 μg/kg), and three high interval doses of ivermectin (400, 200, 200 μg/kg). The primary endpoint of this trial was all-cause of mortality or clinical recovery. The radiographic findings, hospitalization and low O
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Background: Cancer, a major cause of mortality worldwide, is a group of diseases distinguished by uncontrolled growth and expansion of abnormal cells. According to American Cancer Society, melanoma, a kind of skin cancer, is one of the most prevalent cancers. The side effects of chemical treatment developed more demands on natural products. Flavonoids, polyphenol compounds, with anticancer and antioxidant activity attracted more attention to themselves
Materials and Methods: Through this investigation the effect of myricetin on cell proliferation was determined by MTT [Methylthiazolyl diphenyl-tetrazolium bromide] assay. A375 cell lines were seeded in a 96 wells plate and were exposed to different concentrations of myricetin [10, 15, 20, 40, 60, 80, and 100micro MICRO ]. After considered times, the MTT solution was added, then the viability of cells was detected by measuring the absorbance on 570 and 630 nm
Results: Our finding showed that low concentration of myricetin [up to 25micro M] has no toxicity effect. Also the result confirmed the IC[50] of myricetin on melanoma cells for three ordered period [24, 48, 72 hours] as following: 50, 40, 35micro MICRO , respectively
Conclusion: According to this research, myricetin has anti-proliferative effect on melanoma cells, which can be used as a therapeutic agent. We hope that this study could be used as a mile stone in future researches to acquire confirmative results
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Background: in this study, we compared the effect of ibuprofen [IB] while incorporating by Poly Lactic-co-Glycolic Acid [PLGA] nanofiber on expression of adhesion molecules ICAM-1 and VCAM-1 in a mice adhesion model
Materials and Methods: using an adhesion model were induced in mice, PLGA-IB and PLGA membranes and IB were sutured between the abdominal wall and peritoneum after surgical operation to reveal the best membrane for prevention of postoperative adhesion bands by comparison of ICAM-1 and VCAM-1 expression
Results: compared with other groups, PLGA-IB showed a greater ability to reduce ICAM-1 and VCAM-1 expression
Conclusion: these results suggested that in considering the FDA approved polymers, PLGA-IB could be introduced as a potential candidate for prevention of abdominal post-surgery inflammation and adhesion band formation after surgeries
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Background: Rotavirus group A [RVA] is recognized as a major cause of severe gastroenteritis in children and new-born animals. Nonstructural protein 4 [NSP4] is responsible for the enterotoxic activity of these viruses in the villus epithelial cells. Amino acids 114-135 of NSP4 are known to form the diarrhea-inducing region of this viral enterotoxin. Therefore, developing an NSP4 lacking the enterotoxin domain could result in the introduction of a new subunit vaccine against rotaviruses in both humans and animals
Objectives: The aim of this study is the evaluation of rotavirus ANSP4 expression in E. coli expression system before and after removal of the diarrhea-inducing domain, which is the first step towards further immunological studies of the resulting protein
Materials and Methods: Splicing by overlap extension [SOEing] PCR was used to remove the diarrhea-inducing sequence from the NSP4 cDNA. Both the full-length [FL-NSP4] and the spliced [S-NSP4] cDNA amplicons were cloned into pET-32c and pGEX-6P-2. Expression levels of the recombinant proteins were evaluated in E. coli BL21 [DE3] by Western blot analysis. In addition, the toxicity of pET plasmids bearing the S-NSP4 and FL-NSP4 fragments was investigated by plasmid stability test
Results: For FL-NSP4, protein expression was detected for the strain containing the pGEX:FL-NSP4 plasmid, but not for the strain carrying pET:FL-NSP4. Hourly sampling up to 3 h showed that the protein production decreased by time. In contrast, expression of S-NSP4 was detected for pET:S-NSP4 strain, but not for pGEX:S-NSP4. Plasmid stability test showed that pET:S-NSP4 recombinant plasmid was almost stable, while pET:FL-NSP4 was unstable
Conclusions: This is the first report of production of rotavirus NSP4 lacking the diarrhea-inducing domain [S-NSP4]. SNSP4 shows less toxicity in this expression system and potentially could be a promising goal for rotavirus immunological and vaccine studies in the future
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This study discusses the effect of complexes of chitosan grafted polyethylenimine[Ch-PEI] with plasmid DNA on viability of mesenchymal stem cells[MSCs] derived from human marrow. Ch-PEI/pDNA nanoparticles were synthesized through the complex coacervation method using pIRES plasmid containing Green Fluorescent Protein [GFP] gene. To confirm the complexation, samples were run through an agarose gel. Human bone marrow mesenchymal stem cells were studied for the cytotoxicity of the nanoparticles by MTT assay. MTT results indicated Ch-PEI does not have any significant cytotoxicity compared with PEI and Lipofectamine2000 leading to 40% cytotoxicity. According to the results it seems that grafting chitosan with PEI improves the MSCs viability